polyclonal goat anti human jagged1 antibody  (Cell Signaling Technology Inc)

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: Expression of Jagged1 using immunohistochemical and immunofluorescence stains ( A ) in mice in control group ( a ), asthma group ( b ), gamma-secretase inhibitor (DAPT) + ovalbumin (OVA) group ( c ), Mycobacterium vaccae + OVA group ( d ), OVA + M. vaccae group ( e ), and its quantification ( B and C ). Data are reported as mean±SE relative percentage to the control group (n=8). ## P<0.01 vs control; **P <0.01 vs asthma group (ANVOA). Original magnification x400, scale bar=50 μm.

Article Snippet: The tissue sections were incubated in 5% goat serum albumin and subsequently in polyclonal goat anti-human Jagged1 antibody (1:2500; D4Y1R, CST) for 24 h at 4°C,Then, the tissue sections were incubated with the horseradish peroxidase-conjugated secondary antibody for 60 min at room temperature, and subsequently stained with 3,3-diaminobenzidine and counterstained with hematoxylin blue.

Techniques: Expressing, Immunohistochemical staining, Immunofluorescence, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: The mRNA expression of Jagged1 in lung tissues was determined using real-time PCR. Data are reported as mean±SE relative percentage to the control group (n=8). *P<0.05 vs control; # P<0.05 vs asthma group (ANOVA). OVA: ovalbumin; DAPT: gamma-secretase inhibitor.

Article Snippet: The tissue sections were incubated in 5% goat serum albumin and subsequently in polyclonal goat anti-human Jagged1 antibody (1:2500; D4Y1R, CST) for 24 h at 4°C,Then, the tissue sections were incubated with the horseradish peroxidase-conjugated secondary antibody for 60 min at room temperature, and subsequently stained with 3,3-diaminobenzidine and counterstained with hematoxylin blue.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Regulation of γδT17 cells by Mycobacterium vaccae through interference with Notch/Jagged1 signaling pathway

doi: 10.1590/1414-431X20209551

Figure Lengend Snippet: Correlation analysis between Jagged1 and γδT17 cells in lung tissues. A and B , The ratio of IL-17+γδT+ cells to T cells was measured in each group. C , The percentage of IL-17+γδT+ cells and the Jagged1 mRNA expression in lung tissues were positively correlated (r=0.46, P<0.05). Data are reported as mean±SE relative percentage to the control group (n=8). **P<0.01 vs control; *P<0.05, ## P<0.01 vs asthma group (ANOVA). OVA: ovalbumin; DAPT: gamma-secretase inhibitor.

Article Snippet: The tissue sections were incubated in 5% goat serum albumin and subsequently in polyclonal goat anti-human Jagged1 antibody (1:2500; D4Y1R, CST) for 24 h at 4°C,Then, the tissue sections were incubated with the horseradish peroxidase-conjugated secondary antibody for 60 min at room temperature, and subsequently stained with 3,3-diaminobenzidine and counterstained with hematoxylin blue.

Techniques: Expressing, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Inhibition of γ-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma

doi:

Figure Lengend Snippet: Western blot analysis showing expression of Notch1 and Jagged1 in non-neoplastic renal tissue and renal cell carcinoma tissue.

Article Snippet: RAC, antibodies used against Notch1 (polyclonal rabbit anti-human Notch1; ab27526) and Jagged1 (polyclonal goat anti-human Jagged1; sc-6011) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Techniques: Western Blot, Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Inhibition of γ-secretase by retinoic acid chalcone (RAC) induces G2/M arrest and triggers apoptosis in renal cell carcinoma

doi:

Figure Lengend Snippet: Expression of (A) Notch1 and (B) Jagged1 by immunohistochemistry (magnification, × 200).

Article Snippet: RAC, antibodies used against Notch1 (polyclonal rabbit anti-human Notch1; ab27526) and Jagged1 (polyclonal goat anti-human Jagged1; sc-6011) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Techniques: Expressing, Immunohistochemistry

Journal: Japanese journal of clinical oncology

Article Title: High Jagged1 expression predicts poor outcome in clear cell renal cell carcinoma.

doi: 10.1093/jjco/hyq205

Figure Lengend Snippet: Figure 1. Expression of Jagged1 by western blot and reverse transcriptase– polymerase chain reaction (RT–PCR). (a) Protein expression of Jagged1 in renal cell carcinoma (RCC) tissues and non-neoplastic renal tissues by western blot. The level of Jagged1 was higher in RCC tissues than in the adjacent non-neoplastic tissues. (b) The expression of Jagged1 mRNA in RCC tissues and non-neoplastic renal tissues by RT–PCR. The level of expression of Jagged1 was higher in RCC tissues than in the adjacent non- neoplastic tissues. N, non-neoplastic renal tissue; T, renal cell carcinoma tissue. A color version of this figure is available as supplementary data at http://www.jjco.oxfordjournals.org.

Article Snippet: The membrane was blocked with 5% bovine serum albumin in 1 Tris-buffered saline (TBS) buffer at room temperature for 2 h and incubated with polyclonal goat anti-human Jagged1 antibody (1:500; sc-6011, Santa Cruz, CA, USA) at 48C overnight.

Techniques: Expressing, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

Journal: Japanese journal of clinical oncology

Article Title: High Jagged1 expression predicts poor outcome in clear cell renal cell carcinoma.

doi: 10.1093/jjco/hyq205

Figure Lengend Snippet: Figure 2. Expression of Jagged1 by immunohistochemistry. (a and b) Immunohistochemical staining of Jagged1 in RCC tissues and in non- neoplastic renal tissues (magnification 200), respectively. A color version of this figure is available as supplementary data at http://www.jjco. oxfordjournals.org.

Article Snippet: The membrane was blocked with 5% bovine serum albumin in 1 Tris-buffered saline (TBS) buffer at room temperature for 2 h and incubated with polyclonal goat anti-human Jagged1 antibody (1:500; sc-6011, Santa Cruz, CA, USA) at 48C overnight.

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining

Journal: Japanese journal of clinical oncology

Article Title: High Jagged1 expression predicts poor outcome in clear cell renal cell carcinoma.

doi: 10.1093/jjco/hyq205

Figure Lengend Snippet: Figure 3. Overall survival (OS) and disease-free survival (DFS) analysis. (a and b) High Jagged1 expression was significantly associated with reduced OS and DFS, respectively. A color version of this figure is available as supplementary data at http://www.jjco.oxfordjournals.org.

Article Snippet: The membrane was blocked with 5% bovine serum albumin in 1 Tris-buffered saline (TBS) buffer at room temperature for 2 h and incubated with polyclonal goat anti-human Jagged1 antibody (1:500; sc-6011, Santa Cruz, CA, USA) at 48C overnight.

Techniques: Expressing

Journal: Japanese journal of clinical oncology

Article Title: High Jagged1 expression predicts poor outcome in clear cell renal cell carcinoma.

doi: 10.1093/jjco/hyq205

Figure Lengend Snippet: Figure 1. Expression of Jagged1 by western blot and reverse transcriptase– polymerase chain reaction (RT–PCR). (a) Protein expression of Jagged1 in renal cell carcinoma (RCC) tissues and non-neoplastic renal tissues by western blot. The level of Jagged1 was higher in RCC tissues than in the adjacent non-neoplastic tissues. (b) The expression of Jagged1 mRNA in RCC tissues and non-neoplastic renal tissues by RT–PCR. The level of expression of Jagged1 was higher in RCC tissues than in the adjacent non- neoplastic tissues. N, non-neoplastic renal tissue; T, renal cell carcinoma tissue. A color version of this figure is available as supplementary data at http://www.jjco.oxfordjournals.org.

Article Snippet: The specificity of the antibody (polyclonal goat anti-human Jagged1 antibody: sc-6011, Santa Cruz) used in immunoblotting was confirmed by western blot results.

Techniques: Expressing, Western Blot, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

Journal: Japanese journal of clinical oncology

Article Title: High Jagged1 expression predicts poor outcome in clear cell renal cell carcinoma.

doi: 10.1093/jjco/hyq205

Figure Lengend Snippet: Figure 2. Expression of Jagged1 by immunohistochemistry. (a and b) Immunohistochemical staining of Jagged1 in RCC tissues and in non- neoplastic renal tissues (magnification 200), respectively. A color version of this figure is available as supplementary data at http://www.jjco. oxfordjournals.org.

Article Snippet: The specificity of the antibody (polyclonal goat anti-human Jagged1 antibody: sc-6011, Santa Cruz) used in immunoblotting was confirmed by western blot results.

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Staining

Journal: Japanese journal of clinical oncology

Article Title: High Jagged1 expression predicts poor outcome in clear cell renal cell carcinoma.

doi: 10.1093/jjco/hyq205

Figure Lengend Snippet: Figure 3. Overall survival (OS) and disease-free survival (DFS) analysis. (a and b) High Jagged1 expression was significantly associated with reduced OS and DFS, respectively. A color version of this figure is available as supplementary data at http://www.jjco.oxfordjournals.org.

Article Snippet: The specificity of the antibody (polyclonal goat anti-human Jagged1 antibody: sc-6011, Santa Cruz) used in immunoblotting was confirmed by western blot results.

Techniques: Expressing